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6th Flow Cytometry Workshop, Cairo

11th –15th November 2006

Organisers: Professor Azza Kamel, Nahla El Sharkawy, Khaled Shaaban (Cairo) and Michael G. Ormerod (UK)

Friday 10th November- evening

18:30

Registration

 

 

Saturday 11th November

8:15

Registration

 

 

8:45

Welcome

 

 

9:00

(lecture-1)

Introduction to Flow Cytometry

Michael Ormerod

10:30

(lecture-2)

Immunofluorescence and colour compensation

Michael Ormerod

11:30

Coffee break

 

 

12:00

(lecture-3)

Data analysis (including gating)

Michael Ormerod

13:00

(lecture-4)

Methodological issues related to leukemia diagnosis and monitoring

Dario Campana

14:00

Lunch

 

 

15:00

Practical

Group A (Lab)
Introduction to the Flow Cytometer & Colour compensation

Michael Ormerod
Martin Adelmann
Khaled Shaaban

 

 

Group B (Conference room)
Data Introduction and Gating

Nahla El Sharkawy

16:30

 

Group A (Conference room)
Data Introduction and Gating

Khaled Shaaban

 

 

Group B (Lab)
Introduction to the Flow Cytometer & Colour compensation

Michael Ormerod
Martin Adelmann
Nahla El Sharkawy

18:00

Close

 

 

Sunday 12th November

9:00

lecture 1

Antigen quantification

Martin Adelmann

10:00

lecture 2

Modern definition of remission in acute myeloid leukemia

Dario Campana

11:00

Coffee break

 

 

11:30

lecture 3

Determining disease dissemination and response to therapy in lymphoid malignancies

Dario Campana

12:30

lecture 4

Stem cell analysis

Martin Adelmann

13:15

Lecture 5

Quality control

Martin Adelmann

14:00

Lunch

 

 

15:00

Practical

Group A (Lab)
Practical for Antigen quantification and Stem cell analysis

Martin Adelmann
Nahla El Sharkawy
Michael Ormerod

 

 

Group B (Conference room)
Case Presentation including some cases for MRD

Khaled Shaaban
(Campana, Kamel, Schanky)

16:30

 

Group A (Conference room)
Case Presentation including some cases for MRD

Nahla El Sharkawy
(Campana, Kamel, Schanky)

 

 

Group B (Lab)
Practical for Antigen quantification and Stem cell analysis

Martin Adelmann
Khaled Shaaban
Michael Ormerod

18:00

Close

 

 

Monday 13th November

9:15

lecture 1

overview on intracellular antigen staining
Fixation and permeabilization for flow cytometry

Vincent Shankey

10:15

lecture 2

measurement of signal transduction (phospho-epitopes) in human leukemias

Vincent Shankey

11:15

Coffee break

 

 

11:45

lecture 3

The measurement of ZAP-70 in CLL

Vincent Shankey

12:45

lecture 4

Bead-based multiplexed assays

Martin Adelmann

13:45

Lunch

 

 

14:45

Workshop

Group A  (Conference room)

LMD for Bead-based multiplexed assays

Martin Adelmann

 

 

Group B (Lab)

Zap 70 staining of CLL cases

Vincent Shankey

16:00

 

Group B  (Conference room)

LMD for Bead-based multiplexed assays

Martin Adelmann

 

 

Group A (Lab)

Zap 70 staining of CLL cases

Vincent Shankey

17:15

 

LMD for Signal transduction

Vincent Shankey

18:30

Close

 

 

Tuesday 14th November

9:15

lecture 1

DNA Analysis and the cell cycle

Michael Ormerod

10:15

lecture 2

Combining DNA analysis and immunophenotype using DRAQ5

Michael Ormerod

11:15

Coffee break

 

 

11:45

lecture 3

DNA content and cell cycle analysis focus on clinical applications

Vincent Shankey

12:45

lecture 4

Introduction to reticulocyte analysis

Michael Ormerod

13:45

Lunch

 

 

15:00

Practical

Group A (Lab)
Practical for DNA staining

Vincent Schankey
Michael Ormerod
Azza Kamel
Nahla El Sharkawy
Khaled Shaaban

 

 

 

Group B (Conference room)
DNA analysis and presentations

16:30

 

Group B (Lab)
Practical for DNA staining

 

 

Group A (Conference room)
DNA analysis and presentations

18:00

Close

 

 

Wednesday 15th November

9:15

lecture 1

Apoptosis

Michael Ormerod

10:15

lecture 2

measurement of intracellular cytokine

Vincent Shankey

11:15

Coffee break

 

 

11:45

lecture 3

Introduction to Luminex Technology (Instrumentation)

Ieman El Kassas from One Lambda

12:45

lecture 4

XMP technology applications

Ieman El Kassas from One Lambda

13:45

Lunch

 

 

14:45

Workshop

Group A (Lab)
 XMP technology applications in Cytokines estimations

Raghad Safwat
Seham Fouad
Khaled Shaaban

 

 

Group B (Conference room)
LMD for Intracellular antigen staining

Vincent Shankey
Nahla El Sharkawy

16:15

 

Group B (Lab)
 XMP technology applications in Cytokines estimations

Raghad Safwat
Seham Fouad
Nahla El Sharkawy

 

 

Group A (Conference room)
LMD for Intracellular antigen staining

Vincent Shankey
Khaled Shaaban

17:45

 

Round Table Discussion & Certificates ceremony & close of workshop

Azza Kamel

Vincent Shankey

Michael Ormerod
Nahla El Sharkawy
Khaled Shaaban

18:15

Close