7^th Flow Cytometry Workshop, Cairo

Saturday 11^th October

  8:15

Registration

  8:45

Welcome

  9:00

Lecture 1

Introduction to Flow Cytometry

Michael Ormerod

10:45

Lecture 2

Immunofluorescence and colour compensation

Michael Ormerod

11:30

Coffee break

12:00

Lecture 3

Sample preparation and staining, data acquisition

Vera Donnenberg

12:45

Lecture 4

Instrumental set up: calibration and compensation

Vera Donnenberg

13:30

Lunch

14:30

Practical

Group A (Lab)
Introduction to the Flow Cytometer & Colour compensation

Michael Ormerod

Vera Donnenberg
Khaled Shaaban

Group B (Conference room)
Data Introduction and Gating

Nahla El Sharkawy

16:15

Group A (Conference room)
Data Introduction and Gating

Khaled Shaaban

Group B (Lab)
Introduction to the Flow Cytometer & Colour compensation

Michael Ormerod

Vera Donnenberg
Nahla El Sharkawy

18:00

Close

Sunday 12^th October

  9:00

lecture 1

Data analysis, including gating

Michael Ormerod

10:00

lecture 2

Immunophenotyping of leukemia and lymphoma

Paul Wallace

11:00

Coffee break

11:30

lecture 3

Principles of rare event detection

Vera Donnenberg

12:30

lecture 4

Multi-drug resistance

Vera Donnenberg

13:30

Lunch

14:45 – 18:00

Practical

Group A(Lab) Tracking Immune cell function & Proliferation

Paul Wallace

Nahla El-Sharkawy

Azza Kamel, 

14:45 – 18:00

Group B (Conference room)
Case Presentation including some cases for MRD & MDR

Khaled Shaaban
Azza Kamel,

Vera Donnenberg

Monday 13th October

  9:15

lecture 1

Designing flow cytometry experiment

Albert Donnenberg

10:15

lecture 2

Spectral compensation

Albert Donnenberg

11:15

Coffee break

11:45

lecture 3

Image Stream

Paul Wallace

12:45

lecture 4

Imaging flow cytometry

Albert Donnenberg

13:45 -14.45

Lunch

14:45 – 18:00

Workshop

Group B(Lab) Tracking Immune cell function & Proliferation

Paul Wallace

Khaled Shaaban

14:45 – 18:00

Group A(Conference room)
Case Presentation including some cases for MRD & MDR

Nahla El Sharkawy

Azza Kamel,

Vera Donnenberg

Tuesday 14th October

  9:15

lecture 1

Identification of antigen specific T cells

Paul Wallace

10:15

lecture 2

Searching for metastatic tumor in histologically negative bone marrow aspirates

Vera Donnenberg

10:45

Coffee break

11:45

lecture 3

DNA Analysis and the cell cycle

Michael Ormerod

12:45

lecture 4

Combining DNA analysis and immunophenotyping using DRAQ5

Michael Ormerod

13:45

Lunch

15:00

Practical

Group A (Lab)
Practical for DNA staining

Michael Ormerod

Paul Wallace

Nahla El Sharkawy

Group B (Conference room)
DNA analysis and presentations

Khaled Shaaban

Azza Kamel

16:30

Group B (Lab)
Practical for DNA staining

Michael Ormerod

Paul Wallace

Khaled Shaaban,

Group A (Conference room)
DNA analysis and presentations

Nahla El Sharkawy

Azza Kamel

18:00

Close

Wednesday 15th October

  9:15

Lecture 1

Luminex: general technique

Ronald Vos & Francois Topin

10:15

Lecture 2

Luminex Assays

Ronald Vos & Francois Topin

11:15

Coffee break

11:45

lecture 3

Application of HLA on  Luminex

Paul Wong

12:45

Lecture 4

xMAP technology applications

Paul Wong

13:45

Lunch

14:45

Workshop

Group A (Lab)
 xMAP technology applications

16:15

Group B (Lab)
 xMAP technology applications

17:45

Round Table Discussion, Certificates ceremony & close of workshop

18:15

Close